Reporter Synonyms, Reporter Antonyms | vanganthsq.cf

 

define reporter

Define reporter. reporter synonyms, reporter pronunciation, reporter translation, English dictionary definition of reporter. n. 1. A writer, investigator, or presenter of news stories. 2. Law a. A volume containing the published opinions of a court. b. A court official who records. reporter meaning: 1. a person whose job is to discover information about news events and describe them for a newspaper or magazine or for radio or television 2. a person whose job is to discover information about news events and describe them for a newspaper or magazine or for radio or television. The definition of a reporter is a person who gathers information and writes about it.


Reporter - definition of reporter by The Free Dictionary


In molecular biologya reporter gene often simply reporter is a gene that researchers attach to a regulatory sequence of another gene of interest in bacteriacell cultureanimals or plants. Certain genes are chosen as reporters define reporter the characteristics they confer on organisms expressing them are easily identified and measured, define reporter, or because they define reporter selectable markers.

Reporter genes are often used as an indication of whether a certain gene has been taken up by or expressed in the cell or organism population, define reporter. To introduce a reporter gene into an organism, scientists place the reporter define reporter and the gene of interest in the same DNA construct to be inserted into the cell or organism.

For bacteria or prokaryotic cells in culture, define reporter, this is usually in the form of a circular DNA molecule called a plasmid. It is important to use a reporter gene that is not natively expressed in the cell or organism under study, since the expression of the reporter is being used as a marker for successful uptake of the gene of interest.

Commonly used reporter genes that induce visually identifiable characteristics usually involve fluorescent and define reporter proteins. A common reporter in bacteria is the E. This enzyme causes bacteria expressing the gene to appear blue when grown on a medium that contains the substrate analog X-gal.

An example of a selectable-marker which is also a reporter in bacteria is the chloramphenicol acetyltransferase CAT gene, which define reporter resistance to the antibiotic chloramphenicol. Many methods of transfection and transformation — two ways of expressing a foreign or modified gene in an organism — are effective in only a small percentage of a population subjected to the techniques.

Thus, a method for identifying those few successful gene uptake events is necessary. As a result, define reporter, the reporter gene's expression is independent of the gene of interest's expression, which is an advantage when the gene of interest is only expressed under certain specific conditions or in tissues define reporter are difficult to access.

In the case of selectable-marker reporters such as CAT, the transfected population of bacteria can be grown on a substrate that contains chloramphenicol. Only those cells that have successfully taken up the construct containing the CAT gene will survive and multiply under these conditions.

Reporter genes can also be used to assay for the expression of the gene of interest, which may produce a protein that has little obvious or immediate effect on the cell culture or organism. In these cases, the reporter is directly attached to the gene of interest to create a gene fusion.

The two genes are under the same promoter elements and are transcribed into a single messenger RNA molecule. The mRNA is then translated into protein. In these cases it is important that both proteins be able to properly fold into their active conformations and interact with their substrates despite being fused. In building the DNA construct, define reporter, a segment of DNA coding for a flexible polypeptide linker region is usually included so that the reporter and the gene product will only minimally interfere with one define reporter. Reporter gene assay have been increasingly used in high throughput screening HTS to identify small molecule inhibitors and activators of protein targets and pathways for drug discovery and chemical biology.

Because the reporter enzymes themselves e. Reporter genes can be used to assay for the activity of a particular promoter in a cell or organism. The results are normally reported relative to the activity under a "consensus" promoter known to induce strong gene expression. A more complex use of define reporter genes on a large scale is in two-hybrid screeningwhich aims to identify proteins that natively interact with one another in vivo. From Wikipedia, define reporter, the free encyclopedia.

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Reporter dictionary definition | reporter defined

 

define reporter

 

In molecular biology, a reporter gene (often simply reporter) is a gene that researchers attach to a regulatory sequence of another gene of interest in bacteria, cell culture, animals or plants. What does reporter mean? reporter is defined by the lexicographers at Oxford Dictionaries as A person who reports, especially one employed to report news or . A slight gaffe by one of Speaker Gingrich's parents is bound to be pounced upon by the reporter - and other reporters following the story - to open the door wide for a chance to pique the public's interest.